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Covalys Biosciences AG Launches New SNAPtag Kits for Protein Immobilization...



Covalys Biosciences AG, today announced the launch of new products for immobilization and pull-down experiments based on its proprietary SNAP-tag. SNAP-capture kits present a new way to perform pull-down experiments by covalently immobilizing a protein of interest, without prior purification, on a resin or magnetic bead surface. The kits may also be used for studies of protein function.



This novel approach dispenses with the potentially damaging capture of the protein on a chemically activated surface and the problem of drift associated with antibodies for protein capture by offering a universally applicable covalent and orientated immobilization of proteins in physiological conditions. SNAP-capture Magnetic Beads Kits eliminate the need to carry out repeated centrifugation steps thus facilitating processing of samples and minimizing sample loss. SNAP-capture Purification is intended for protein purification and permits immobilization under mild conditions, followed by vigorous washing of the immobilized protein without fear of eluting the protein of interest as the interaction with the resin is covalent. A new addition to the building blocks product range CBG-NH2 offers a further improved way to immobilize proteins to carboxy surfaces for applications such as Biacore.



SNAP-express Gateway plasmids offer a fast and easy way to insert the ORF of interest from a Gateway Entry Clone into a mammalian SNAP-express Gateway destination vector thus minimizing or obviating the time required for cloning. SNAP-express Gateway plasmids are available for cloning the ORF to either the N or C terminus of the SNAP-tag and are supplied with a positive control which directs a SNAP-tag fusion protein to the nucleus.



The introduction of the MCP-tag offers a novel and highly effective way to specifically label proteins on the surface of living cells. Once the protein of interest is expressed as a MCP-tag fusion it can be rapidly covalently labeled by exposing the cells to a non-cell permeable fluorescent CoA Substrate in the presence of a specific labeling enzyme, SFP-Synthase. This enables precise control of the timing of onset of fluorescence. In contrast to the autofluorescent proteins, the same fusion protein can be labeled with different fluorophores simply by exposing the cells to different fluorescent substrates. The small MCP-tag can be used in combination with ACP-tag to visualize two different fusion proteins on the surface of living cells by sequentially labeling proteins with CoA Substrates carrying different fluorophores and the appropriate Synthases (also available from Covalys). Labeling is highly specific and is restricted to the cell surface therefore there is no background associated with internal pools of proteins. New CoA substrates for labeling ACP-tag and MCP-tag include CoA-Biotin for selective biotinylation of cell surface proteins and CoA-SH, a building block containing a thiol group that enables customers to make their own CoA Substrates.



"The ability to rapidly, specifically, and covalently immobilize proteins of interest for pull down assays facilitates the study of protein-protein interactions particularly in the functional genomics area and will simplify and improve the quality of sample processing," said Dr. Andreas Brecht, Chief Executive Officer at Covalys. "We are also very excited to be able to offer the SNAP-tag in an expression vector with Invitrogen Corporations' Gateway technology to enable our customers to rapidly and simply generate their constructs using this advanced cloning system that is a standard in the market. Furthermore, the introduction of MCP-tag when used together with the ACP-tag permits sophisticated experimental approaches as two different fusion proteins can be visualized simultaneously on cell surfaces by virtue of these small protein tags."



All Covalys technologies are available with an implicit license for use. For details please see http://www.covalys.com/technical-resources/limited-use-label-licences.html



About Covalys Biosciences AG

Covalys provides products that help researchers in academic institutions and pharmaceutical and biotech companies to improve the efficiency of research into proteins and their function. Its SNAP-tag technology is the first commercially available protein tag which covalently links itself to any chemical probe provided as a substrate. ACP-tag allows site-specific labeling of proteins on cell surfaces. Covalys' technologies are exclusively licensed from the Swiss Federal Institute of Technology Lausanne (EPFL). Founded in June 2002, Covalys has headquarters in Witterswil, Switzerland. For more information, visit Covalys' website: www.covalys.com



Trademarks: SNAP-tag is a registered trademark of Covalys Biosciences AG. Gateway is a registered trademark of Invitrogen Corporation. Biacore is a registered trademark of GE Healthcare.



Media Contact:

Andreas Brecht

Covalys Biosciences AG

Phone +41 (0)61 725 20 50






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